# ## this is a configuration file for processingUnique.sam script # ## it will contain all the necessary informations for # ## AS event file name ## data type (single-end or paired-end) ## base of the experiment ## list of sam files ## etc.. ## # #################################### ### readLength ### -readLength = 72 # #################################### ### junctionLength: it can be calculated as ### junctionLength = 2*(readLength-anchorLength) # ### For example, if your readLength is 50 and you ran tophat with -a/--min-anchor-length 8 ### junction length becomes 2*(50-8) = 84 # -junctionLength = 142 # ##################################### ### AS file name ### AS event file names ### it currently support 5 AS event types ### full path here # -SE = /dfs2/cbcl/forouzme/Projects/PolyA/rMATS/rMATS.3.2.5/colleen-cort-ctrl-KO-mm9-frstrand/ASEvents/fromGTF.SE.txt -MXE = /dfs2/cbcl/forouzme/Projects/PolyA/rMATS/rMATS.3.2.5/colleen-cort-ctrl-KO-mm9-frstrand/ASEvents/fromGTF.MXE.txt -A5SS = /dfs2/cbcl/forouzme/Projects/PolyA/rMATS/rMATS.3.2.5/colleen-cort-ctrl-KO-mm9-frstrand/ASEvents/fromGTF.A5SS.txt -A3SS = /dfs2/cbcl/forouzme/Projects/PolyA/rMATS/rMATS.3.2.5/colleen-cort-ctrl-KO-mm9-frstrand/ASEvents/fromGTF.A3SS.txt -RI = /dfs2/cbcl/forouzme/Projects/PolyA/rMATS/rMATS.3.2.5/colleen-cort-ctrl-KO-mm9-frstrand/ASEvents/fromGTF.RI.txt # #################################### ### experiment name -experiment = RNASeq # #################################### ### base name for sample_1 and sample_2 -base_1 = SAMPLE_1 -base_2 = SAMPLE_2 # ###################################### ### data type ### single-end or paired-end # #-dataType = single #-dataType = paired -dataType = paired # ###################################### ### library type ### fr_unstranded, fr_firststrand, fr_secondstrand # #-libType = fr-unstranded #-libType = fr-firststrand #-libType = fr-secondstrand -libType = fr-firststrand ###################################### ### sam file folder ### user can put all sam files in the same place (probably using "symbolic link") # -samDir = ###################################### ### out file folder ### MATS input file will be in this folder # -outDir = /dfs2/cbcl/forouzme/Projects/PolyA/rMATS/rMATS.3.2.5/colleen-cort-ctrl-KO-mm9-frstrand/temp # ##################################### ### sam file name ### #-input_1 = unique.ESRP.1.sam -input_1 = /dfs2/cbcl/forouzme/Projects/PolyA/rMATS/rMATS.3.2.5/colleen-cort-ctrl-KO-mm9-frstrand/SAMPLE_1/REP_1/aligned.sorted.bam,/dfs2/cbcl/forouzme/Projects/PolyA/rMATS/rMATS.3.2.5/colleen-cort-ctrl-KO-mm9-frstrand/SAMPLE_1/REP_2/aligned.sorted.bam,/dfs2/cbcl/forouzme/Projects/PolyA/rMATS/rMATS.3.2.5/colleen-cort-ctrl-KO-mm9-frstrand/SAMPLE_1/REP_3/aligned.sorted.bam,/dfs2/cbcl/forouzme/Projects/PolyA/rMATS/rMATS.3.2.5/colleen-cort-ctrl-KO-mm9-frstrand/SAMPLE_1/REP_4/aligned.sorted.bam #-input_2 = unique.EV.1.sam -input_2 = /dfs2/cbcl/forouzme/Projects/PolyA/rMATS/rMATS.3.2.5/colleen-cort-ctrl-KO-mm9-frstrand/SAMPLE_2/REP_1/aligned.sorted.bam,/dfs2/cbcl/forouzme/Projects/PolyA/rMATS/rMATS.3.2.5/colleen-cort-ctrl-KO-mm9-frstrand/SAMPLE_2/REP_2/aligned.sorted.bam,/dfs2/cbcl/forouzme/Projects/PolyA/rMATS/rMATS.3.2.5/colleen-cort-ctrl-KO-mm9-frstrand/SAMPLE_2/REP_3/aligned.sorted.bam,/dfs2/cbcl/forouzme/Projects/PolyA/rMATS/rMATS.3.2.5/colleen-cort-ctrl-KO-mm9-frstrand/SAMPLE_2/REP_4/aligned.sorted.bam ################################### ### alert email ### email address to receive pipleline progress (error, warning, or end of process) ### change this to your email address -email = yourmail@domain.com # #